EFFECT OF LOCALLY INJECTED ANTI-INFLAMMATORY DRUGS ON SYNTHESIS OF COLLAGEN AND NON-COLLAGEN PROTEIN OF CARRAGEENIN GRANULOMA IN RATS

The effect of steroid and non-steroid anti-inflammatory drugs was studied on protein synthesis of the granulation tissue induced by carrageenin on the dorsum of rats. The drugs were injected daily into the pouch of preformed granuloma for 4 days from day 5 up to day 8. Immediately after the last injection of the drugs the rats were injected s.c. with 3H-proline, and 24 hr later the granuloma was harvested on day 9. The total content, specific activity and total radioactivity of collagen and non-collagen protein of the granuloma were determined. Betamethasone disodium phosphate, a steroid drug, markedly inhibited the incorporation of 3H-proline into collagen hydroxyproline as well as into non-collagen protein. Among the non-steroids tested, indomethacin and phenylbutazone failed to show any significant changes in the synthesis of these proteins. Sodium salicylate significantly reduced the synthesis of collagen in the granulomatous tissue without affecting non-collagen protein synthesis. © 1969.