STIMULATION OF PROTEIN-SYNTHESIS BY RAISED EXTRACELLULAR PH IN CARDIAC MYOCYTES AND PERFUSED HEARTS

被引：18

作者：

GAITANAKI, CJ ^{[1
]}

SUGDEN, PH ^{[1
]}

FULLER, SJ ^{[1
]}

机构：

[1] UNIV LONDON, NATL HEART & LUNG INST, DEPT CARDIAC MED, DOVEHOUSE ST, LONDON SW3 6LY, ENGLAND

来源：

FEBS LETTERS | 1990年 / 260卷 / 01期

关键词：

(Rat heart); Bicarbonate buffer; Hepes buffer; Intracellular pH; Protein synthesis;

D O I：

10.1016/0014-5793(90)80061-M

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Protein synthesis was stimulated in freshly-isolated rat cardiac myocytes by increasing the extracellular pH of Hepes-buffered Tyrode's solutions over the range pH 7.4-8.4. The maximal stimulation was about 45%. Protein synthesis in anterogradely-perfused rat hearts was stimulated by 11% by increasing the pH of the bicarbonate-containing perfusion medium from pH 7.4 to 7.8. This manoeuvre increased intracellular pH by 0.12 units. A concomitant increase in phosphocreatine concentration was observed. These findings are consistent with the hypothesis that intracellular pH may exert profound effects on tissue protein synthesis rates. © 1990.

引用

页码：42 / 44

页数：3

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