MOLECULAR-WEIGHT SEPARATION OF PROTEINS AND PEPTIDES WITH A NEW HIGH-PRESSURE LIQUID-CHROMATOGRAPHY COLUMN

被引：26

作者：

GRUBER, KA

WHITAKER, JM

MORRIS, M

机构：

[1] Department of Physiology and Pharmacology, Bowman Gray School of Medicine, Winston-Salem

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 97卷 / 01期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/0003-2697(79)90343-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new high-pressure liquid molecular weight chromatography column was evaluated for its ability to separate proteins and peptides. The column was able to give a linear separation of compounds between 5,000-700,000 Mr. Chromatography of posterior pituitary extracts, tumor-associated fetal antigens, and estrogen receptors demonstrated the ability of the column to separate biological samples. © 1979.

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页码：176 / 183

页数：8

相关论文

共 18 条

[1] AUTOMATIC MONITORING OF PRIMARY AMINES IN PREPARATIVE COLUMN EFFLUENTS WITH FLUORESCAMINE [J].

BOHLEN, P ;

STEIN, S ;

STONE, J ;

UDENFRIEND, S .

ANALYTICAL BIOCHEMISTRY, 1975, 67 (02) :438-445

[2]

EVANS DL, CANCER RES

[3] DISSOCIATION OF OXYTOCIN AND VASOPRESSIN FROM THEIR CARRIER PROTEIN BY CHROMATOGRAPHY ON SEPHADEX G-25 [J].

FRANKLAND, BT ;

HOLLENBERG, MD ;

HOPE, DB ;

SCHACTER, BA .

BRITISH JOURNAL OF PHARMACOLOGY AND CHEMOTHERAPY, 1966, 26 (02) :502-+

[4] NEUROPHYSIN BIOSYNTHESIS - CONVERSION OF A PUTATIVE PRECURSOR DURING AXONAL-TRANSPORT [J].

GAINER, H ;

SARNE, Y ;

BROWNSTEIN, MJ .

SCIENCE, 1977, 195 (4284) :1354-1356

[5] SEPARATION OF NEUROHYPOPHYSEAL PROTEINS BY REVERSED-PHASE HIGH-PRESSURE LIQUID-CHROMATOGRAPHY [J].

GLASEL, JA .

JOURNAL OF CHROMATOGRAPHY, 1978, 145 (03) :469-472

[6]

GRUBER KA, 1978, P SOC EXP BIOL MED, V159, P463

[7] FLUOROMETRIC ASSAY OF VASOPRESSIN AND OXYTOCIN - GENERAL APPROACH TO ASSAY OF PEPTIDES IN TISSUES [J].

GRUBER, KA ;

STEIN, S ;

BRINK, L ;

RADHAKRISHNAN, A ;

UDENFRIEND, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1976, 73 (04) :1314-1318

[8]

GRUBER KA, 1979, FED P ABSTR 11086

[9]

KUTE TE, 1978, CANCER RES, V38, P4307

[10] HIGH-EFFICIENCY CATION-EXCHANGE CHROMATOGRAPHY OF POLYPEPTIDES AND POLYAMINES IN NANOMOLE RANGE [J].

RADHAKRISHNAN, AN ;

STEIN, S ;

LICHT, A ;

GRUBER, KA ;

UDENFRIEND, S .

JOURNAL OF CHROMATOGRAPHY, 1977, 132 (03) :552-555