EXPRESSION OF THE DROSOPHILA-MELANOGASTER METALLOTHIONEIN GENES IN YEAST

被引：10

作者：

SILAR, P ^{[1
]}

WEGNEZ, M ^{[1
]}

机构：

[1] UNIV PARIS 11, EMBRYOL MOLEC & EXPTL LAB,CNRS,UNITE D1134, BATIMENT 445, F-91405 ORSAY, FRANCE

来源：

FEBS LETTERS | 1990年 / 269卷 / 01期

关键词：

Drosophila melanogaster; Metal-resistance; Metallothionein; Transformation;

D O I：

10.1016/0014-5793(90)81172-K

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The metallothionein system in Drosophila melanogaster is composed of two genes, Mto and Mn, that code for distinctly different proteins. In order to compare the properties of Mto and Mtn, we transformed yeast with several fusion plasmids. The Mto and Mtn cDNAs, when placed under the control of CUP1 or PGK promoters, can confer a copper-resistance phenotype to copper-hypersensitive cells. Both Mto and Mtn proteins can be characterized in extracts from transformed yeast cells. © 1990.

引用

页码：273 / 276

页数：4

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