IMPROVED FIXATION OF FROZEN LYMPHO-HEMATOPOIETIC TISSUE-SECTIONS WITH HEXAZOTIZED PARAROSANILINE

被引：25

作者：

DEJONG, JP ^{[1
]}

VOERMAN, JSA ^{[1
]}

LEENEN, PJM ^{[1
]}

VANDERSLUIJSGELLING, AJ ^{[1
]}

PLOEMACHER, RE ^{[1
]}

机构：

[1] ERASMUS UNIV,DEPT CELL BIOL,POB 1738,3000 DR ROTTERDAM,NETHERLANDS

来源：

HISTOCHEMICAL JOURNAL | 1991年 / 23卷 / 09期

关键词：

D O I：

10.1007/BF01042295

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

In this study, a simple single-step fixation method for frozen tissue sections is introduced using the hexazotized salt of Pararosaniline as preservative agent. Tissue preservation by this method was shown to be superior to the commonly-used fixation with acetone. Fixation with hexazotized Pararosaniline caused a minimal loss of antigenicity as demonstrated using twenty-three monoclonal antibodies directed against lympho-haemopoietic and stromal cells.

引用

页码：392 / 401

页数：10

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