CONFOCAL IMAGING WITH BILATERAL SCANNING AND ARRAY DETECTORS

被引:60
作者
BRAKENHOFF, GJ
VISSCHER, K
机构
[1] Department of Molecular Cell Biology, University of Amsterdam, Amsterdam, 1018 TV
来源
JOURNAL OF MICROSCOPY-OXFORD | 1992年 / 165卷
关键词
BILATERAL; CONFOCAL; FLUORESCENCE; MICROSCOPY; SECTIONING; REAL-TIME; CCD; ARRAY DETECTOR; LASER;
D O I
10.1111/j.1365-2818.1992.tb04311.x
中图分类号
TH742 [显微镜];
学科分类号
摘要
A novel arrangement for confocal microscopy is presented, in which the key elements are the use of an array detector such as a CCD for confocal image collection and the use of one double-sided scanning mirror element for bilaterally scanning the object and collecting the data on the CCD. The resulting arrangement is shown to be capable of confocal imaging with high photon efficiency under adjustable conditions of confocality and varying image acquisition rates, i.e. from slow speed up to real-time imaging. Either laser or conventional light sources may be utilized. In addition to CCD registration, direct observation by eye of the confocal image in fluorescence is also possible.
引用
收藏
页码:139 / 146
页数:8
相关论文
共 13 条
  • [1] OBSERVATION OF A SINGLE-BEAM GRADIENT FORCE OPTICAL TRAP FOR DIELECTRIC PARTICLES
    ASHKIN, A
    DZIEDZIC, JM
    BJORKHOLM, JE
    CHU, S
    [J]. OPTICS LETTERS, 1986, 11 (05) : 288 - 290
  • [2] BRAKENHOFF GJ, 1989, P SOC PHOTO-OPT INS, V1139, P117, DOI 10.1117/12.961783
  • [3] CONFOCAL SCANNING LIGHT-MICROSCOPY WITH HIGH APERTURE IMMERSION LENSES
    BRAKENHOFF, GJ
    BLOM, P
    BARENDS, P
    [J]. JOURNAL OF MICROSCOPY-OXFORD, 1979, 117 (NOV): : 219 - 232
  • [4] 3-DIMENSIONAL CHROMATIN DISTRIBUTION IN NEURO-BLASTOMA NUCLEI SHOWN BY CONFOCAL SCANNING LASER MICROSCOPY
    BRAKENHOFF, GJ
    VANDERVOORT, HTM
    VANSPRONSEN, EA
    LINNEMANS, WAM
    NANNINGA, N
    [J]. NATURE, 1985, 317 (6039) : 748 - 749
  • [5] BRAKNEHOFF GJ, 1978, P ICO, V2, P215
  • [6] 3-DIMENSIONAL MICROSCOPY USING A CONFOCAL LASER SCANNING MICROSCOPE
    CARLSSON, K
    DANIELSSON, PE
    LENZ, R
    LILJEBORG, A
    MAJLOF, L
    ASLUND, N
    [J]. OPTICS LETTERS, 1985, 10 (02) : 53 - 55
  • [8] TANDEM-SCANNING REFLECTED-LIGHT MICROSCOPE
    PETRAN, M
    HADRAVSKY, M
    EGGER, MD
    GALAMBOS, R
    [J]. JOURNAL OF THE OPTICAL SOCIETY OF AMERICA, 1968, 58 (05) : 661 - +
  • [9] SHEPPARD CJR, 1978, P ICO, V2, P211
  • [10] VANDERVOORT HTM, 1988, P SPIE, V1028, P39