DEVELOPMENTAL-CHANGES IN RAT-LIVER MITOCHONDRIAL POPULATIONS ANALYZED BY FLOW-CYTOMETRY

被引：22

作者：

LOPEZMEDIAVILLA, C

ORFAO, A

MIGUEL, JS

MEDINA, JM

机构：

[1] UNIV SALAMANCA,FAC FARM,DEPT BIOQUIM & BIOL MOLEC,APDO 449,E-37080 SALAMANCA,SPAIN

[2] UNIV SALAMANCA,SERV CITOMETRIA,SALAMANCA,SPAIN

[3] UNIV SALAMANCA,HOSP CLIN,DEPT HEMATOL,SALAMANCA,SPAIN

来源：

EXPERIMENTAL CELL RESEARCH | 1992年 / 203卷 / 01期

关键词：

D O I：

10.1016/0014-4827(92)90048-D

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Isolated rat liver mitochondria were split into three density fractions when applied to a Percoll gradient. This phenomenon is observable in the fetus, in the early newborn (1 h), in the suckling newborn (7 days), and in the adult, suggesting that the three density fractions coexist regardless of the state of development. The medium-density fraction sharply decreased immediately after delivery, being replaced by the high-density fraction. Flow cytometry analysis of mitochondrial density fractions stained with rhodamine 123 showed the occurrence in each density fraction and in all developmental states studied of two distinct mitochondrial populations with different fluorescence intensities. Our results suggest that the high-fluorescence population might be an immature form of mitochondria that decreases with the progression of development, coinciding with the postnatal enhancement of mitochondrial respiratory efficiency. © 1992.

引用

页码：134 / 140

页数：7

共 23 条

[1] POSTNATAL-DEVELOPMENT OF RAT-LIVER MITOCHONDRIA - STATE-3 RESPIRATION, ADENINE-NUCLEOTIDE TRANSLOCASE ACTIVITY, AND THE NET ACCUMULATION OF ADENINE-NUCLEOTIDES [J].