PRESYNAPTIC ENHANCEMENT OF EXCITATORY SYNAPTIC TRANSMISSION BY BETA-ADRENERGIC-RECEPTOR ACTIVATION

被引:68
作者
GEREAU, RW
CONN, PJ
机构
[1] EMORY UNIV,SCH MED,NEUROSCI PROGRAM,ATLANTA,GA 30322
[2] EMORY UNIV,SCH MED,DEPT PHARMACOL,ATLANTA,GA 30322
关键词
D O I
10.1152/jn.1994.72.3.1438
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
1. Previous studies have shown that beta-adrenergic receptor activation has many effects on neuronal function in hippocampal area CA1. However, all of the physiological effects of beta-adrenergic receptor activation in this region reported to date have been attributed to postsynaptic mechanisms. A series of studies was performed to test the hypothesis that beta-adrenergic receptor activation also acts presynaptically to enhance excitatory synaptic transmission. 2. Application of the selective beta-adrenergic agonist isoproterenol to hippocampal slices induced an increase in the amplitude of evoked excitatory postsynaptic currents (EPSCs) in CA1 pyramidal cells. This response was potentiated in the presence of a cyclic nucleotide phosphodiesterase inhibitor. Isoproterenol also resulted in the appearance of a late inward synaptic current that likely represents polysynaptically evoked EPSCs. Both the increased amplitude of the monosynaptic EPSC and the appearance of polysynaptic EPSCs in response to isoproterenol were blocked by H89, an inhibitor of adenosine 3',5'-cyclic monophosphate (cAMP)-dependent protein kinase. 3. Isoproterenol induced an increase in the frequency of spontaneous miniature EPSCs but did not affect the amplitude of these currents. In addition, isoproterenol had no effect on currents elicited by direct application of the ionotropic glutamate receptor agonist, (R,S)-alpha-amino-3-hydroxy-5-methylisoxazol-4-propionic acid (AMPA). 4. These results suggest that activation of presynaptic beta-adrenergic receptors enhances synaptic transmission in area CA1 via activation of cAMP-dependent protein kinase.
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页码:1438 / 1442
页数:5
相关论文
共 16 条
[1]   WHOLE CELL RECORDING FROM NEURONS IN SLICES OF REPTILIAN AND MAMMALIAN CEREBRAL-CORTEX [J].
BLANTON, MG ;
LOTURCO, JJ ;
KRIEGSTEIN, AR .
JOURNAL OF NEUROSCIENCE METHODS, 1989, 30 (03) :203-210
[2]  
BROWNING M D, 1990, Society for Neuroscience Abstracts, V16, P653
[3]  
CHAVEZNORIEGA LE, 1994, J NEUROSCI, V14, P310
[4]   MODULATION OF SYNAPTIC EFFICACY IN FIELD CA1 OF THE RAT HIPPOCAMPUS BY FORSKOLIN [J].
CHAVEZNORIEGA, LE ;
STEVENS, CF .
BRAIN RESEARCH, 1992, 574 (1-2) :85-92
[5]  
DUNWIDDIE TV, 1992, J NEUROSCI, V12, P506
[6]  
GEREAU RW, 1994, J NEUROSCI, V14, P3310
[7]   NORADRENALINE AND BETA-ADRENOCEPTOR AGONISTS INCREASE ACTIVITY OF VOLTAGE-DEPENDENT CALCIUM CHANNELS IN HIPPOCAMPAL-NEURONS [J].
GRAY, R ;
JOHNSTON, D .
NATURE, 1987, 327 (6123) :620-622
[8]   ENHANCEMENT OF THE GLUTAMATE RESPONSE BY CAMP-DEPENDENT PROTEIN-KINASE IN HIPPOCAMPAL-NEURONS [J].
GREENGARD, P ;
JEN, J ;
NAIRN, AC ;
STEVENS, CF .
SCIENCE, 1991, 253 (5024) :1135-1138
[9]  
HEGINBOTHAM LR, 1991, J NEUROSCI, V11, P2519
[10]   NORADRENERGIC INNERVATION OF THE ADULT-RAT HIPPOCAMPAL-FORMATION [J].
LOY, R ;
KOZIELL, DA ;
LINDSEY, JD ;
MOORE, RY .
JOURNAL OF COMPARATIVE NEUROLOGY, 1980, 189 (04) :699-710