INTRACELLULAR LOCATION OF INAPPARENTLY INFECTING CHLAMYDIA IN SYNOVIAL TISSUE FROM PATIENTS WITH REITERS-SYNDROME

被引:48
作者
BEUTLER, AM
WHITTUMHUDSON, JA
NANAGARA, R
SCHUMACHER, HR
HUDSON, AP
机构
[1] DEPT VET AFFAIRS MED CTR, MED RES SERV, PHILADELPHIA, PA 19104 USA
[2] DEPT VET AFFAIRS MED CTR, CTR ARTHRIT IMMUNOL, PHILADELPHIA, PA 19104 USA
[3] UNIV PENN, SCH MED, DEPT MED, DIV RHEUMATOL, PHILADELPHIA, PA 19104 USA
[4] MED COLL PENN, DEPT MICROBIOL & IMMUNOL, PHILADELPHIA, PA 19129 USA
[5] JOHNS HOPKINS UNIV, SCH MED, WILMER INST, BALTIMORE, MD 21205 USA
关键词
CHLAMYDIA; REITERS SYNDROME; IN SITU HYBRIDIZATION; SYNOVIA;
D O I
10.1007/BF02918277
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Culture of Chlamydia trachomatis from synovial tissues/fluids from Reiter's syndrome (RS) patients frequently yields negative results. However, we have identified chlamydial RNA at that site in such patients, suggesting that viable organisms may be present. Here we define the cellular location of chlamydia within the synovium via in situ hybridization. Using a chlamydial ribosomal RNA-directed probe, we show that synovial tissue from culture-negative RS patients gives strong hybridization which is often localized to a subsynovial cell layer, rather than to the synovial lining; in some cases, hybridizing cells are dispersed through the synovium. Ah hybridization signal is located within host cells, indicating that infectious extracellular elementary bodies are rare or absent. These data confirm the extensive intracellular presence of inapparent chlamydia in the synovia of RS patients and provide some insight into the usual culture negativity of synovial tissues for the organism.
引用
收藏
页码:163 / 171
页数:9
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