INFLUENCE OF INTRACELLULAR RENIN ON HEART CELL COMMUNICATION

被引：47

作者：

DEMELLO, WC ^{[1
]}

机构：

[1] UNIV PUERTO RICO,SCH MED,DEPT PHARMACOL & ANESTHESIOL,SAN JUAN,PR 00936

来源：

HYPERTENSION | 1995年 / 25卷 / 06期

关键词：

RENIN; HEART; CELL COMMUNICATION;

D O I：

10.1161/01.HYP.25.6.1172

中图分类号：

R6 [外科学];

学科分类号：

1002 ; 100210 ;

摘要：

The influence of intracellular renin and angiotensinogen on the control of cell-to-cell communication in heart muscle was investigated in cell pairs isolated from adult rat ventricle. Junctional conductance was measured with two separated rated voltage-clamp circuits. Intracellular dialysis of renin (0.2 pmol/L) caused a decrease in junctional conductance of 29+/-3.8% (+/-SEM, P<.05) in 7 minutes. The effect of renin on junctional conductance seems to be mainly due to the synthesis of Ang II because enalaprilat (10(-9) mol/L) dialyzed into the cell caused an appreciable reduction in the effect of renin. The intracellular administration of renin (0.2 pmol/L) plus angiotensinogen (0.4 pmol/L) produced a faster and stronger fall in junctional conductance (84.3+/-1.35%, P<.05), and the effect was greatly reduced by enalaprilat. The effects of both renin and angiotensinogen on junctional conductance were not related to a fall in surface cell membrane resistance or a change in series resistance. The effect of renin on junctional conductance was blocked by intracellular administration of a renin inhibitor (S 2864). Moreover, renin dialyzed into just one cell of the pair induced rectification of the junctional membrane, which was prevented by enalaprilat. The results support the view that an intracrine renin-angiotensin system in the heart regulates intercellular communication.

引用

页码：1172 / 1177

页数：6

共 31 条

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