SYNERGISTIC EFFECT OF ACUTE-HYPOXIA ON FLOW-INDUCED RELEASE OF ATP FROM CULTURED ENDOTHELIAL-CELLS

被引:94
作者
BODIN, P [1 ]
BURNSTOCK, G [1 ]
机构
[1] UCL, DEPT ANAT & DEV BIOL, LONDON WC1E 6BT, ENGLAND
来源
EXPERIENTIA | 1995年 / 51卷 / 03期
关键词
VASCULAR ENDOTHELIAL CELLS; CULTURES; ATP; ACUTE HYPOXIA; PERFUSION; INCREASED FLOW;
D O I
10.1007/BF01931108
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Human umbilical vein endothelial cells (HUVECs) in primary cultures were perfused under normoxic or hypoxic conditions. These cells were stimulated twice for 3 min by increased flow (from 0.5 to 3.0 ml/min). Under hypoxic conditions the basal release of ATP was the same as under normoxic conditions, but during increased flow the release was greater (0.58 +/- 0.07 > 0.32 +/- 0.04 pmoles/ml/10(6) cells ( +78%), for the first period of stimulation; 0.39 +/- 0.05 > 0.22 +/- 0.03 pmoles/ml/10(6) cells ( +79%) for the second period). Further experiments with sequential increments in flow rate showed that under both normoxic and hypoxic conditions, a positive correlation existed between ATP release and the rate of flow but there was always more ATP released under hypoxic conditions regardless of the flow rate. HUVECs in secondary culture (second passage) were similarly stimulated. No differences were observed between normoxic and hypoxic conditions. In both cases, the quantity of ATP released during high flow (0.050 +/- 0.004 pmoles/ml/10(6) cells) was significantly smaller than the quantity of ATP released during low flow (0.09 +/- 0.01 pmoles/ml/10(6) cells). To conclude, since hypoxia alone did not affect ATP release, there appears to be a synergistic relationship between increased shear stress and hypoxia in the stimulation of ATP release from HUVECs. Moreover, the release of ATP under these conditions seems to be a property of highly differentiated endothelial cells.
引用
收藏
页码:256 / 259
页数:4
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