SCREENING OF CYCLIC PEPTIDE PHAGE LIBRARIES IDENTIFIES LIGANDS THAT BIND STREPTAVIDIN WITH HIGH AFFINITIES

被引:176
作者
GIEBEL, LB [1 ]
CASS, RT [1 ]
MILLIGAN, DL [1 ]
YOUNG, DC [1 ]
ARZE, R [1 ]
JOHNSON, CR [1 ]
机构
[1] ARRIS PHARMACEUT CORP,DEPT COMBINATORIAL CHEM,S SAN FRANCISCO,CA 94080
关键词
D O I
10.1021/bi00047a006
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The screening of combinatorial peptide libraries has emerged as an important tool in the discovery of novel substrates or ligands for enzyme and receptor targets. For example, screening linear peptide Libraries using streptavidin as a model receptor system has previously identified many low-affinity peptide ligands, all of which contain the common motif His-Pro-Gin (HPQ). We reasoned that constraining the conformational freedom of linear peptides by cyclization in a library would yield peptide ligands of increased affinity. Three different cyclic peptide libraries were constructed in an M13 phage display system as N-terminal pIII protein fusions. The random peptide sequences were flanked by two cysteine residues, which allows efficient disulfide bond formation and cyclization during phage assembly. These cyclic peptide libraries were screened with streptavidin as the model receptor system. Many sequences, all of which contained the motif His-Pro-Gln (HPQ), were discovered, and in the preceding paper, the structures of complexes of streptavidin-bound cyclic and linear peptides are described (Katz, 1995). Analysis of binding kinetics and affinities demonstrated that the conformationally constrained cyclic peptides bound streptavidin with affinities up to 3 orders of magnitude higher than linear peptides identified in previous library screens. These results demonstrate the potential of screening conformationally constrained peptide libraries for high-affinity novel receptor ligands or enzyme substrates.
引用
收藏
页码:15430 / 15435
页数:6
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