MULTIPLE DOMAINS MEDIATE HEME CONTROL OF THE YEAST ACTIVATOR HAP1

被引：19

作者：

HALDI, ML ^{[1
]}

GUARENTE, L ^{[1
]}

机构：

[1] MIT,DEPT BIOL,CAMBRIDGE,MA 02139

来源：

MOLECULAR & GENERAL GENETICS | 1995年 / 248卷 / 02期

关键词：

YEAST; TRANSCRIPTION; HAP1; HEME;

D O I：

10.1007/BF02190805

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The activity of the yeast activator HAP1 in vivo requires heme. A heme responsive domain of HAP1 was identified previously. It is adjacent to the DNA-binding domain, and appears to block DNA binding in the absence of heme. Here we describe a novel genetic selection which yielded mutants of HAP1 that are independent of heme when assayed for activation. These mutants define a second region of HAP1, close to the activation domain, which also controls its response to heme.

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页码：229 / 235

页数：7

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