ANTIBODY AGAINST THE C-TERMINAL PORTION OF DYSTROPHIN CROSS-REACTS WITH THE 400 KDA PROTEIN IN THE PIA MATER OF DYSTROPHIN-DEFICIENT MDX MOUSE-BRAIN

The mdx mouse is an animal model for X-linked Duchenne muscular dystrophy. A polyclonal antibody against a synthetic peptide IV equivalent to the C-terminal portion (amino acids 3495-3544) of dystrophin crossreacted with a 400 kDa protein in the brain and the spinal cord of mdx mouse, as well as in the control B10 mouse. However, the protein did not crossreact with the polyclonal antibody raised against the N-terminal portion of dystrophin peptide I (amino acids 215-264). Immunofluorescent micrography revealed that the outside of the small arteries and the pia mater of the brain strongly reacted with the anti-peptide IV antibody. These results strongly suggest the presence of a crossreactive protein other than dystrophin, possibly a dystrophin-related autosomal gene product, in the pia mater. © 1990 COPYRIGHT, 1990 BY The Journal of Biochemistry.