EFFECT OF AUXIN AND CYTOKININ ON NEWLY SYNTHESIZED PROTEINS OF CULTURED DOUGLAS-FIR COTYLEDONS

Newly synthetized cyloplasmic proteins of Douglas fir cotyledon culture capable of initiating adventitious bud formation (bud culture) were compared with those of morphologically distinct types of cultures, bud‐callus culture (some adventitious buds with callus), and callus culture (no adventitious buds; only callus). SDS polyacrylamide gel electrophoretic profiles of double labeled (14C‐and 3H‐) proteins showed that a persistent increase of low molecular weight proteins (16,000 to 20,000 daltons) was associated with bud culture. Similar electrophoretic profiles of proteins extracted in the absence and in the presence of protease inhibitor (4‐mcthylbenzene sulfonyl fluoride) or phenolic compounds absorbents (Dowex I‐X8 and polyvinylpolypyrolidine) were obtained indicating that the detected low molecular weights of these proteins represented the real monomeric state of these molecules. Copyright © 1979, Wiley Blackwell. All rights reserved