ENHANCING EFFECT OF MAGNESIUM-ION ON CELL-FREE SYNTHESIS OF READ-THROUGH PROTEIN OF BACTERIOPHAGE-Q-BETA

被引：18

作者：

HIRASHIMA, A

HARIGAI, H

WATANABE, I

机构：

[1] Department of Molecular Biology, Keio University, School of Medicine, Shinjuku-ku, Tokyo

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 88卷 / 03期

关键词：

D O I：

10.1016/0006-291X(79)91514-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This report describes the enhancing effect of magnesium ion on the synthesis of read-through protein of bacteriophage Qβ in a cell-free protein synthesizing system from E. coli. At 6 mM of magnesium acetate, the major product was coat protein. At 12 mM of magnesium, it was replaced by read-through protein. This enhanced synthesis was substituted by the addition of 0.25 mM of spermine or 1 mM of spermidine to 6 mM of magnesium. These results suggest that magnesium or combination of magnesium and polyamines causes leaky termination at the end of the coat protein cistron of Qβ-RNA. © 1979.

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页码：1046 / 1051

页数：6

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