ORAL IMMUNIZATION OF RATS WITH PROTEINOID MICROSPHERES ENCAPSULATING INFLUENZA-VIRUS ANTIGENS

被引：46

作者：

SANTIAGO, N ^{[1
]}

MILSTEIN, S ^{[1
]}

RIVERA, T ^{[1
]}

GARCIA, E ^{[1
]}

ZAIDI, T ^{[1
]}

HONG, H ^{[1
]}

BUCHER, D ^{[1
]}

机构：

[1] NEW YORK MED COLL, DEPT MICROBIOL & IMMUNOL, VALHALLA, NY 10595 USA

来源：

PHARMACEUTICAL RESEARCH | 1993年 / 10卷 / 08期

关键词：

ORAL IMMUNIZATION; MICROSPHERES; PROTEINOID; INFLUENZA ANTIGENS;

D O I：

10.1023/A:1018992924025

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Influenza virus antigen microspheres were prepared by a pH-dependent process using a protein-like polymer (proteinoid) made by thermal condensation of amino acids. The efficacy of these preparations to induce specific IgG responses when used as oral vaccines in rats was evaluated. A single enteric dose of M1 entrapped in proteinoid microspheres was able to induce a significant IgG response to MI as early as 2 weeks postdosing, while rats dosed orally with the same M1 total dose (no microspheres) showed no detectable antibody response. An unencapsulated hemagglutinin and neuraminidase (HA-NA) preparation induced a moderate anti HA-NA IgG response. A single enteric dose of HA-NA spheres induced a response in 33% of the rats; this response was up to eight times higher than that observed in the rats dosed with unencapsulated antigen.

引用

页码：1243 / 1247

页数：5

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