3-DIMENSIONAL CRYSTAL-STRUCTURE OF RECOMBINANT ERABUTOXIN-A AT 2.0 ANGSTROM RESOLUTION

被引：12

作者：

ARNOUX, B

MENEZ, R

DREVET, P

BOULAIN, JC

DUCRUIX, A

MENEZ, A

机构：

[1] CEA SACLAY,CE SACLAY,DSV,DEPT INGN & ETUD PROT,F-91191 GIF SUR YVETTE,FRANCE

[2] LAB BIOL STRUCT,CNRS,F-91198 GIF SUR YVETTE,FRANCE

来源：

FEBS LETTERS | 1994年 / 342卷 / 01期

关键词：

RECOMBINANT SNAKE TOXIN; CRYSTAL STRUCTURE;

D O I：

10.1016/0014-5793(94)80574-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recombinant erabutoxin a (Ea(r)) has been crystallized by vapour diffusion in hanging drops. The crystals belong to space group P2(1)2(1)2(1) with cell dimensions a = 55.8 Angstrom, b = 53.4 Angstrom, c = 40.8 Angstrom. Diffraction data have been recorded on a FAST detector up to 2.0 Angstrom. The atomic crystal structure of Ea(r) has been determined by initial refinement of the structure of the isotoxin erabutoxin b (Eb) the crystals of which were grown under identical conditions. The R-factor was 23% at 2.0 Angstrom resolution. The secondary and tertiary structures of Ea, are shown to be identical with that of wild-type Eb, within the experimental error.

引用

页码：12 / 14

页数：3

共 13 条

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