IMPROVEMENT OF A MONOCLONAL ANTIBODY-BASED IMMUNOASSAY FOR THE DETERMINATION OF TERBUTRYN

The performance of an existing enzyme immunoassay (EIA) with monoclonal antibodies (mAb) for the determination of terbutryn was improved by the application of a new enzyme tracer. For this purpose the triazine derivative 6-chloro-2-(tert-butylamino)-1,3,5-triazine-4-(6-amino-hexane carboxylic acid) was coupled to horseradish peroxidase (HRP). The competitive EIA on microwell plates made it possible to determine terbutryn in the range from 0.05 to 1 mu g/L with a 50% B/B-0 value of the test at 0.2 mu g/L. The application of the EIA to determine terbutryn in spiked surface waters provided good recoveries of terbutryn without matrix effects.