IDENTIFICATION AND CHARACTERIZATION OF THE DEVELOPMENTALLY-REGULATED PATTERN OF EXPRESSION IN THE TESTIS OF A MOUSE GENE EXHIBITING SIMILARITY TO THE FAMILY OF PHOSPHODIESTERASES

A cDNA for a rat brain phosphodiesterase (PDE) was used to screen a mouse testis library to identify the murine PDEs which are expressed in this tissue. A clone of 981 bp, p4-6, was isolated and shown to exhibit limited identity at the amino acid level to the rat brain PDE (20%). The putative protein encoded by clone p4-6 also contains multiple potential modification sites, for phosphorylation, myristylation, and glycosylation, many of which are located at positions similar to those found for rat brain PDE. The gene identified by p4-6 yields 3 transcripts, an abundant 1.9 kb transcript, and less abundant transcripts of 3.8 and 6.7 kb. Of the nine tissues examined in this study, the expression of the corresponding gene was limited to the adult mouse testis. Furthermore, the expression in the testis was most abundant in the germ cell lineage, although low levels were detected in somatic cells of the testis as well. Analysis of RNA from testes at different stages of development suggested that the p4-6 gene is most abundantly expressed in germ cells that have completed the meiotic divisions.