INTERACTION OF A LIMITED SET OF PROTEINS WITH DIFFERENT MESSENGER-RNAS AND PROTECTION OF 5'-CAPS AGAINST PYROPHOSPHATASE DIGESTION IN INITIATION-COMPLEXES

被引:32
作者
SONENBERG, N
MORGAN, MA
TESTA, D
COLONNO, RJ
SHATKIN, AJ
机构
[1] Roche Institute of Molecular Biology, Nutley
关键词
D O I
10.1093/nar/7.1.15
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A variety of 5′- 3H-methyl-labeled, oxidized viral mRNAs were used as probes for detecting in wheat germ initiation complexes proteins that interact with, and can be cross-linked to, the 5′-cap structure. A limited and reproducible set of specific proteins was obtained with the different mRNAs. The binding of these proteins to the 5′-end of mRNA apparently results in protection against nucleotide pyrophosphatase digestion of the cap even in initiation complexes in which the 5′-end is susceptible to pancreatic RNase digestion. Cross-linked proteins from mammalian initiation complexes comigrated with several of the subunits of similarly treated eIF-3. A model for cap binding protein interaction with mRNA cap during initiation of translation is suggested. © 1979 Information Retrieval Limited.
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页码:15 / 29
页数:15
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