INVITRO HYDROXYLATION OF BENTAZON BY MICROSOMES FROM NAPHTHALIC ANHYDRIDE-TREATED CORN SHOOTS

被引：61

作者：

MCFADDEN, JJ ^{[1
]}

GRONWALD, JW ^{[1
]}

EBERLEIN, CV ^{[1
]}

机构：

[1] USDA ARS,PLANT SCI RES UNIT,ST PAUL,MN 55108

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 168卷 / 01期

关键词：

D O I：

10.1016/0006-291X(90)91695-O

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In vitro metabolism of the herbicide bentazon was studied in microsomal membranes isolated from 6-day-old etiolated com shoots. Microsomes isolated from shoots of nontreated seeds did not metabolize bentazon when assayed with NADPH or peroxides. However, microsomes isolated from shoots of seeds pretreated with naphthalic anhydride formed a single bentazon metabolite when provided with NADPH. The metabolite was identified as 6-hydroxybentazon, the major phase I metabolite produced in vivo. In vitro formation of this metabolite was strongly inhibited by carbon monoxide, nitrogen, and tetcyclacis (10 μM). The results suggest that aryl hydroxylation of bentazon in com shoots is catalyzed by a cytochrome P-450 (E.C. 1.14.14.1) and that a seed pretreatment with naphthalic anhydride is necessary for recovery of activity in vitro. © 1990.

引用

页码：206 / 213

页数：8

共 25 条

[1] INDUCTION OF CYTOCHROME-P-450 AND MONO-OXYGENASE ACTIVITY BY 2,4-DICHLOROPHENOXYACETIC ACID IN HIGHER-PLANT TISSUE [J].