BACKBONE-MODIFIED OLIGONUCLEOTIDES CONTAINING A BUTANEDIOL-1,3 MOIETY AS A VICARIOUS SEGMENT FOR THE DEOXYRIBOSYL MOIETY - SYNTHESIS AND ENZYME STUDIES

被引：17

作者：

WILK, A

KOZIOLKIEWICZ, M

GRAJKOWSKI, A

UZNANSKI, B

STEC, WJ

机构：

[1] Polish Academy of Sciences, Centre of Molecular and Macromolecular Studies

来源：

NUCLEIC ACIDS RESEARCH | 1990年 / 18卷 / 08期

关键词：

D O I：

10.1093/nar/18.8.2065

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sequential single replacement of nucleosides within the decanucleotide d[GGGAATTCCC] (7) by means of a butanediol-1,3 residue allowed us to obtain a set of ten decanucleotides containing 'vicarious' (V) carbonphosphate fragments. These analogues were further used as substrates for svPDE, nuclease PI and EcoR1 endonuclease. Interestingly, replacement of any of the nucleosides within the canonical sequence ...GAATTC... by the butanediol-1,3 'vicarious' segment discriminates such constructs 8c-h as substrates for Eco-R1 enzyme. © 1990 Oxford University Press.

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页码：2065 / 2068

页数：4

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