THE PROMOTER OF THE GLUCOAMYLASE-ENCODING GENE OF ASPERGILLUS-NIGER FUNCTIONS IN USTILAGO-MAYDIS

被引：19

作者：

SMITH, TL

GASKELL, J

BERKA, RM

YANG, M

HENNER, DJ

CULLEN, D

机构：

[1] US FOREST SERV,INST MICROBIAL & BIOCHEM TECHNOL,FOREST PROD LAB,MADISON,WI 53705

[2] US FOREST SERV,INST MICROBIAL & BIOCHEM TECHNOL,FOREST PROD LAB,MADISON,WI 53705

[3] UNIV WISCONSIN,CTR BIOTECHNOL,MADISON,WI 53706

[4] GENENCOR INC,S SAN FRANCISCO,CA 94080

[5] GENENTECH INC,DEPT CELL GENET,S SAN FRANCISCO,CA 94080

来源：

GENE | 1990年 / 88卷 / 02期

关键词：

Ascomycete; Basidiomycete; fungal transformation; Hygromycin phosphotransferase; recombinant DNA; starch;

D O I：

10.1016/0378-1119(90)90040-X

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Promoter sequences from the Aspergillus niger glucoamylase-encoding gene (glaA) were linked to the bacterial hygromycin (Hy) phosphotransferase-encoding gene (hph) and this chimeric marker was used to select Hy-resistant (hyR) Ustilago maydis transformants. This is an example of an Ascomycete promoter functioning in a Basidiomycete. HyR transformants varied with respect to copy number of integrated vector, mitotic stability, and tolerance to Hy. Only 216 bp of glaA promoter sequence is required for expression in U. maydis but this promoter is not induced by starch as it is in Aspergillus spp. The transcriptional start points are the same in U. maydis and A. niger. © 1990.

引用

页码：259 / 262

页数：4

共 14 条

[1] 2 DIFFERENT TYPES OF INTERVENING SEQUENCES IN THE GLUCOAMYLASE GENE FROM ASPERGILLUS-NIGER [J].