CULTURED VASCULAR ENDOTHELIAL-CELLS SECRETE A PROTEIN FACTOR(S) THAT PROMOTES THE CONTRACTION OF COLLAGEN LATTICES MADE WITH FIBROBLASTS

被引：5

作者：

CHEN, JK ^{[1
]}

GREEN, G ^{[1
]}

WEINBERG, CB ^{[1
]}

机构：

[1] ORGANOGENESIS INC,CAMBRIDGE,MA 02142

来源：

EXPERIMENTAL CELL RESEARCH | 1991年 / 193卷 / 02期

关键词：

D O I：

10.1016/0014-4827(91)90100-9

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Conditioned media collected from arterial endothelial cells contain protein factor(s) that promotes the contraction of collagen lattices made with skin fibroblasts. Based on the lattice contraction-promoting activity, a protein with an apparent molecular weight of 22 kDa was identified. This 22-kDa protein stimulated lattice contraction in both serum-containing and serum-free media. When assayed at a 30% equivalent of the conditioned medium, the contraction-promoting activity of the purified factor was about 50 to 60% of that elicited by the unfractionated conditioned medium. Some contraction-promoting activity was also present in certain subfractions of the conditioned medium generated during the separation of the 22-kDa protein. Taken together, the results indicate that the lattice contraction-promoting activity in the endothelial cell-conditioned medium is probably aided by multiple active principles. The biochemical and biological characteristics indicated that the 22-kDa protein is not a transforming growth factor-β-related factor nor a fibroblast growth promoter. © 1991.

引用

页码：297 / 302

页数：6

共 27 条

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[2] PRODUCTION OF A TISSUE-LIKE STRUCTURE BY CONTRACTION OF COLLAGEN LATTICES BY HUMAN-FIBROBLASTS OF DIFFERENT PROLIFERATIVE POTENTIAL INVITRO [J].