ELECTROCHEMICAL INVESTIGATION OF INTERMOLECULAR ELECTRON-TRANSFER BETWEEN 2 PHYSIOLOGICAL PARTNERS - CYTOCHROME-C3 AND IMMOBILIZED HYDROGENASE FROM DESULFOVIBRIO-DESULFURICANS NORWAY

被引：13

作者：

DRAOUI, K

BIANCO, P

HALADJIAN, J

GUERLESQUIN, F

BRUSCHI, M

机构：

[1] UNIV AIX MARSEILLE 1,CHIM & ELECTROCHIM COMPLEXES LAB,CNRS,CHIM BACTERIENNE LAB,CASE 57,F-13331 MARSEILLE 3,FRANCE

[2] CNRS,CHIM BACTERIENNE LAB,F-13277 MARSEILLE 9,FRANCE

来源：

JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 1991年 / 313卷 / 1-2期

关键词：

D O I：

10.1016/0022-0728(91)85180-W

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The modified pyrolytic graphite electrode resulting from the adsorption of hydrogenase from Desulfovibrio desulfuricans Norway on the graphite surface is used to study the interaction between hydrogenase and its physiological redox partner cytochrome c3. The kinetics of the system hydrogenase/cytochrome c3 are investigated using cyclic voltammetry. It is shown that the catalytic current depends on several factors such as the ionic strength and pH. By varying the cytochrome c3 concentration a value of 3-mu-M is obtained for the "apparent" Michaelis-Menten constant. The comparison with other cytochromes c3 shows that a poor current is detected for the system Desulfovibrio desulfuricans Norway hydrogenase/Desulfovibrio gigas cytochrome c3.

引用

页码：201 / 214

页数：14

共 37 条

[1] ADAMS MWW, 1981, BIOCHIM BIOPHYS ACTA, V594, P105
[2] CURRENT-POTENTIAL RESPONSES FOR A TETRAHEMIC PROTEIN - A METHOD OF DETERMINING THE INDIVIDUAL HALF-WAVE POTENTIALS OF CYTOCHROME-C3 FROM DESULFOVIBRIO-DESULFURICANS STRAIN NORWAY
BIANCO, P
HALADJIAN, J
[J]. ELECTROCHIMICA ACTA, 1981, 26 (08) : 1001 - 1004
[3] THE MECHANISM OF THE ELECTRON-TRANSFER REACTIONS OF CYTOCHROME-C3 FROM DESULFOVIBRIO-DESULFURICANS NORWAY AT A MERCURY-ELECTRODE
BIANCO, P
HALADJIAN, J
[J]. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1987, 223 (1-2): : 201 - 214
[4] PROMOTION OF THE POLAROGRAPHIC-REDUCTION OF FERREDOXINS BY DIVALENT METAL-CATIONS - EFFECT OF PH
BIANCO, P
HALADJIAN, J
[J]. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1986, 199 (02): : 365 - 379
[5] AN ELECTROCHEMICAL STUDY OF THE STABILITY OF CYTOCHROME-C3 FROM DESULFOVIBRIO-DESULFURICANS NORWAY STRAIN
BIANCO, P
HALADJIAN, J
BRUSCHI, M
[J]. BIOELECTROCHEMISTRY AND BIOENERGETICS, 1986, 15 (01): : 57 - 66
[6] CORRELATIONS STUDIES BETWEEN STRUCTURAL AND REDOX PROPERTIES OF CYTOCHROMES-C3
BRUSCHI, M
LOUTFI, M
BIANCO, P
HALADJIAN, J
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1984, 120 (02) : 384 - 389
[7] PURIFICATION AND CHARACTERIZATION OF CYTOCHROME C-3, FERREDOXIN, AND RUBREDOXIN ISOLATED FROM DESULFOVIBRIO-DESULFURICANS NORWAY
BRUSCHI, M
HATCHIKIAN, CE
GOLOVLEVA, LA
LEGALL, J
[J]. JOURNAL OF BACTERIOLOGY, 1977, 129 (01) : 30 - 38
[8] ELECTRON-SPIN-RESONANCE STUDIES OF CYTOCHROME-C3 FROM DESULFOVIBRIO-DESULFURICANS STRAIN NORWAY-4 - MIDPOINT POTENTIALS OF THE 4 HEMES, AND INTERACTIONS WITH FERREDOXIN AND COLLOIDAL SULFUR
CAMMACK, R
FAUQUE, G
MOURA, JJG
LEGALL, J
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1984, 784 (01) : 68 - 74
[9] REDOX PROTEIN ELECTRON-TRANSFER MECHANISMS - ELECTROSTATIC INTERACTIONS AS A DETERMINANT OF REACTION SITE IN C-TYPE CYTOCHROMES
CHEDDAR, G
MEYER, TE
CUSANOVICH, MA
STOUT, CD
TOLLIN, G
[J]. BIOCHEMISTRY, 1989, 28 (15) : 6318 - 6322
[10] DETERMINATION OF THE MACROSCOPIC FORMAL POTENTIALS OF CYTOCHROME-C3 OF DESULFOVIBRIO-VULGARIS, MIYAZAKI-F, THROUGH THE COMBINED USE OF H-1-NMR AND AN OPTICALLY TRANSPARENT THIN-LAYER ELECTRODE CELL
FAN, K
AKUTSU, H
NIKI, K
HIGUCHI, N
KYOGOKU, Y
[J]. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1990, 278 (1-2) : 295 - 306

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