ELECTROCHEMICAL INVESTIGATION OF INTERMOLECULAR ELECTRON-TRANSFER BETWEEN 2 PHYSIOLOGICAL PARTNERS - CYTOCHROME-C3 AND IMMOBILIZED HYDROGENASE FROM DESULFOVIBRIO-DESULFURICANS NORWAY

被引:13
作者
DRAOUI, K
BIANCO, P
HALADJIAN, J
GUERLESQUIN, F
BRUSCHI, M
机构
[1] UNIV AIX MARSEILLE 1,CHIM & ELECTROCHIM COMPLEXES LAB,CNRS,CHIM BACTERIENNE LAB,CASE 57,F-13331 MARSEILLE 3,FRANCE
[2] CNRS,CHIM BACTERIENNE LAB,F-13277 MARSEILLE 9,FRANCE
来源
JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 1991年 / 313卷 / 1-2期
关键词
D O I
10.1016/0022-0728(91)85180-W
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The modified pyrolytic graphite electrode resulting from the adsorption of hydrogenase from Desulfovibrio desulfuricans Norway on the graphite surface is used to study the interaction between hydrogenase and its physiological redox partner cytochrome c3. The kinetics of the system hydrogenase/cytochrome c3 are investigated using cyclic voltammetry. It is shown that the catalytic current depends on several factors such as the ionic strength and pH. By varying the cytochrome c3 concentration a value of 3-mu-M is obtained for the "apparent" Michaelis-Menten constant. The comparison with other cytochromes c3 shows that a poor current is detected for the system Desulfovibrio desulfuricans Norway hydrogenase/Desulfovibrio gigas cytochrome c3.
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页码:201 / 214
页数:14
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