MOLECULAR-CLONING OF THE TRYPTOPHAN OPERON FROM AN AEROMONAS-HYDROPHILA FRESH-WATER ISOLATE

被引：1

作者：

ANGUITA, J ^{[1
]}

RIVERO, O ^{[1
]}

NAHARRO, G ^{[1
]}

机构：

[1] UNIV LEON,FAC VET,DEPT PATOL ANIM SANIDAD ANIM,UNIDAD MICROBIOL,E-24071 LEON,SPAIN

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1992年 / 58卷 / 03期

关键词：

D O I：

10.1128/AEM.58.3.1031-1034.1992

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A genomic library of Aeromonas hydrophila F9 was constructed by using pBR322 as a vector. From that, two DNA fragments (5.8 and 11.6 kb) were isolated containing genetic information to complement trpA and trpB defects (5.8-kb fragment) and to complement trpA, trpB, trpC, trpD, and trpE defects (11.6-kb fragment) in Escherichia coli mutants. Evidence of the existence of a secondary promoter is given.

引用

页码：1031 / 1034

页数：4

共 24 条

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