RAPID FETAL KARYOTYPE FROM CYSTIC HYGROMA AND PLEURAL EFFUSIONS

被引：8

作者：

COSTA, D ^{[1
]}

BORRELL, A ^{[1
]}

MARGARIT, E ^{[1
]}

CARRIO, A ^{[1
]}

SOLER, A ^{[1
]}

BALMES, I ^{[1
]}

ESTIVILL, X ^{[1
]}

FORTUNY, A ^{[1
]}

机构：

[1] UNIV BARCELONA,HOSP CLIN BARCELONA,DEPT OBSTET & GYNECOL,E-08036 BARCELONA,SPAIN

来源：

PRENATAL DIAGNOSIS | 1995年 / 15卷 / 02期

关键词：

PRENATAL DIAGNOSIS; RAPID KARYOTYPE; PLEURAL EFFUSION; CYSTIC HYGROMA;

D O I：

10.1002/pd.1970150206

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Fluid from pleural effusion (n=2) and cystic hygroma (n=7) was obtained from eight fetuses, between 13 and 32 weeks of pregnancy at the time when a conventional prenatal diagnosis procedure was carried out. As these fluids contain lymphocytes, they were processed like peripheral blood. A karyotype was obtained in 4 days in both cases of pleural effusion and in four out of seven samples of cystic hygroma. An abnormal karyotype was detected in three of the four samples of cystic hygroma: two trisomies 21 and a monosomy X. Different parameters were evaluated in order to predict the feasibility of obtaining a cytogenetic diagnosis. Our data showed that if the amount of fluid obtained was greater than or equal to 4 ml and the initial lymphocyte count (ILC) was >0.2 x 10(6) cells/ml a cytogenetic diagnosis was possible from an initial concentration of cultured lymphocytes (ICCL) of >0.06 x 10(6) cells/ml.

引用

页码：141 / 148

页数：8

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