THIN-LAYER GEL FILTRATION OF PROTEINS .I. METHOD

Several aspects of thin-layer gel filtration have been re-examined. By the use of standard densitometric equipment, a quantitative evaluation of the thin-layer gel filtration pattern has been made possible. The recovery of proteins by the print technique has been studied using a number of proteins of different molecular weights. For three Sephadex gels, G-75, G-100 and G-200, a linear correlation was found between the RM value (defined as the ratio of the migration distance of the tested protein to that of myoglobin, which was used as a standard) and the molecular weight or the molecular radius of a number of globular proteins. Fibrinogen fitted well into the straight line of the RM-log molecular radius relationship, but not into that of the RM-log molecular weight relationship. Apoferritin did not fit into the RM-log molecular weight relationship and its molecular radius of 80 Å is in conflict with values found by the other methods. On Bio-Gel P-300 ferritin was separated into three fractions which on disc electrophoresis were found to differ in the content of the electrophoretically distinct α, β and γ-components. © 1968.