RELEASE OF LIPOPROTEIN LIPASE FROM FAT CELLS

被引:19
作者
STEWART, JE
WHELAN, CF
SCHOTZ, MC
机构
[1] Radioisotope Research, Veterans Administration Center, Los Angeles
[2] Department of Biological Chemistry, UCLA Center for the Health Sciences, Los Angeles
[3] Department of Oral Diagnosis, UCLA Center for the Health Sciences, Los Angeles
关键词
D O I
10.1016/0006-291X(69)90391-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
When isolated fat cells, prepared from rat epididymal pads, were incubated, increasing amounts of lipase activity were found in the cell-free medium. Most of this lipase activity had characteristics of lipoprotein lipase. Increasing the temperature that fat cells were incubated from 23° to 37° markedly increased the appearance of lipase activity in the medium. In contrast, the appearance of this enzyme activity was markedly decreased when cells were incubated in the presence of cyanide. The data presented suggest that the release of lipoprotein lipase activity on incubation of fat cells is an energy-dependent process. © 1969.
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页码:376 / &
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