CHROMATOGRAPHIC FRACTIONATION OF ESCHERICHIA-COLI TRANSFER-RNA ON A NEW SUPPORT, NAPHTHOYL-SEPHAROSE

被引：9

作者：

HJERTEN, S ^{[1
]}

HELLMAN, U ^{[1
]}

SVENSSON, I ^{[1
]}

ROSENGREN, J ^{[1
]}

机构：

[1] PHARM FINE CHEM AB,DEPT RES,S-75104 UPPSALA,SWEDEN

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1979年 / 1卷 / 05期

关键词：

hydrophobic interaction; Sepharose reversed gradient chromatography; tRNA fractionation;

D O I：

10.1016/0165-022X(79)90001-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The noncharged naphthoyl-Sepharose CL-6B has been prepared. Escherichia coli tRNA binds to this new adsorbent in 0.75 M ammonium sulphate at neutral pH at room temperature. Using a negative salt gradient, the tRNA are eluted in a defined order. The chromatographic pattern is clearly different from those of other commonly used tRNA separation techniques. © 1979.

引用

页码：263 / 273

页数：11

共 21 条

[1] STUDIES ON MICROBIAL RNA - FRACTIONATION OF TRNA METHYLASES FROM SACCHAROMYCES CEREVISIAE [J].