POTENTIOMETRIC METHOD FOR SUBSTRATE ANALYSIS USING IMMOBILIZED NAD+-DEPENDENT OXIDOREDUCTASE ENZYMES

被引：13

作者：

CHEN, AK

LIU, CC

SCHILLER, JG

机构：

[1] CASE WESTERN RESERVE UNIV,DEPT CHEM ENGN,CLEVELAND,OH 44106

[2] UNIV PITTSBURGH,DEPT MED TECHNOL,PITTSBURGH,PA 15261

[3] UNIV PITTSBURGH,DEPT CHEM & PETR ENGN,PITTSBURGH,PA 15261

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1979年 / 21卷 / 11期

关键词：

D O I：

10.1002/bit.260211103

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Two coenzyme‐dependent oxidoreductases, glucose dehydrogenase and alcohol dehydrogenase, were immobilized in polyacrylamide gel over a platinum grid matrix and used as enzyme electrodes to measure their substrate concentrations in buffered aqueous solutions. The immobilized enzymes were used to oxidize their substrates in the presence of NAD+. Ferricyanide was used as the redox mediator and electroactive specific. The determinations of glucose and ethenol were utilized to demonstrate and evaluate the performance of the system. The described methodology should be readily applicable to the analysis of numerous other substrates of coenzyme‐dependent oxidoreductases. Copyright © 1979 John Wiley & Sons, Inc.

引用

页码：1905 / 1915

页数：11

共 8 条

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[2] Schiller J.G., Chen A.K., Liu C.C., Anal. Biochem., 85, (1978)
[3] Chen A.K., Liu C.C., Biotechnol. Bioeng., 19, (1977)
[4] Blaedel W.J., Kessel T.R., Boguslaski R.C., Anal. Chem., 44, (1972)
[5] Metzger R.P., Wilcox S.S., Wick A.N., J. Biol. Chem., 240, (1965)
[6] Bergmeyer H.U., Methods of Enzymatic Analysis, 1, (1974)
[7] Bergmeyerm H.U., Methods of Enzymatic Analysis, 1, (1974)
[8] Kuan J.W., Kuan S.S., Guilbault G.G., Anal. Chem., 23, (1977)

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