CORRECT SPLICING OF MODIFIED INTRONS OF A RHIZOPUS PROTEINASE GENE IN SACCHAROMYCES-CEREVISIAE

被引：3

作者：

ASHIKARI, T ^{[1
]}

AMACHI, T ^{[1
]}

YOSHIZUMI, H ^{[1
]}

HORIUCHI, H ^{[1
]}

TAKAGI, M ^{[1
]}

YANO, K ^{[1
]}

机构：

[1] UNIV TOKYO,DEPT AGR CHEM,BUNKYO KU,TOKYO 113,JAPAN

来源：

MOLECULAR & GENERAL GENETICS | 1990年 / 223卷 / 01期

关键词：

Rhizopus aspartic proteinase; Saccharomyces cerevisiae; Splicing of intron;

D O I：

10.1007/BF00315791

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The intron of the Rhizopus aspartic proteinase gene (RNAP-I) was modified by in vitro mutagenesis and examined for its splicing efficiency in Saccharomyces cerevisiae. The wild-type intron of the RNAP-I gene was not spliced at all in spite of its structural similarity to introns of S. cerevisiae. The primary transcript of the RNAP-I gene was converted to correctly translatable mRNA only when the complete consensus sequence of S. cerevisiae introns (i.e. 5″-GTATGT-----TACTAAC-----TAG-3″) was introduced into its intron, although the efficiency of splicing was low. It is also shown that transformants carrying the RNAP-I gene with the complete consensus sequence of S. cerevisiae introns produce active RNAP-I protein. © 1990 Springer-Verlag.

引用

页码：11 / 16

页数：6

共 26 条

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[2]

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