INFLUENCE OF TEMPERATURE ON THE SEPARATION PROPERTIES OF IMMOBILIZED CARBOWAX-20M OPEN TUBULAR COLUMN LIQUID-CHROMATOGRAPHY

被引：7

作者：

JANAK, K ^{[1
]}

HORKA, M ^{[1
]}

KREJCI, M ^{[1
]}

机构：

[1] CZECHOSLOVAK ACAD SCI,INST ANALYT CHEM,KOUNICOVA 42,CS-61142 BRNO,CZECHOSLOVAKIA

来源：

JOURNAL OF MICROCOLUMN SEPARATIONS | 1991年 / 3卷 / 03期

关键词：

OPEN TUBULAR COLUMNS; IMMOBILIZED CARBOWAX-20M; GLASS TRANSITION TEMPERATURE; STATIONARY PHASE DIFFUSIBILITY;

D O I：

10.1002/mcs.1220030306

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Properties of Carbowax 20M stationary phase immobilized with pluriisocyanates were investigated in the temperature interval 20-80-degrees-C. Phase transition was observed under both GC and LC conditions. Depending on the type of crosslinking agent, different phase transition temperature intervals were found with the same end point transition temperature of 55-degrees-C in the GC system and 60-degrees-C in the LC system. In contrast to GC, the retention behavior of the columns in the LC system is not significantly influenced by phase transition. A severe drop of the height equivalent to a theoretical plate was observed near the phase transition in both systems. It was due to the increase in diffusivity of the stationary phase.

引用

页码：203 / 211

页数：9

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