HERPES-SIMPLEX VIRUS-DNA ISOLATION FROM INFECTED-CELLS WITH A NOVEL PROCEDURE

被引：105

作者：

PIGNATTI, PF

CASSAI, E

MENEGUZZI, G

CHENCINER, N

MILANESI, G

机构：

[1] CNR,IST GENET,PAVIA,ITALY

[2] CNR,GENET BIOCHIM & EVOLUZ LAB,PAVIA,ITALY

[3] UNIV FERRARA,IST MICROBIOL,I-44100 FERRARA,ITALY

[4] FAC SCI NICE,CTR BIOCHIM,F-06034 NICE,FRANCE

来源：

VIROLOGY | 1979年 / 93卷 / 01期

关键词：

D O I：

10.1016/0042-6822(79)90295-2

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Herpes Simplex Virus type 1-infected cells were extracted in the presence of 0.25% Triton X-100-0.2 M NaCl. Viral DNA associated with proteins was found in the supernatant after low-speed centrifugation. Only viral DNA was recovered by this procedure, as shown by CsCl density analysis after deproteinization. Full-length viral DNA molecules were observed in the electron microscope. © 1979.

引用

页码：260 / 264

页数：5

共 10 条

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