HUMAN URINE STIMULATES GROWTH OF LEISHMANIA INVITRO

被引：60

作者：

HOWARD, MK ^{[1
]}

PHAROAH, MM ^{[1
]}

ASHALL, F ^{[1
]}

MILES, MA ^{[1
]}

机构：

[1] UNIV LONDON IMPERIAL COLL SCI & TECHNOL,DEPT PURE & APPL BIOL,LONDON SW7 2AZ,ENGLAND

来源：

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE | 1991年 / 85卷 / 04期

基金：

英国惠康基金;

关键词：

D O I：

10.1016/0035-9203(91)90226-O

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Enhancement of trypanosomatid metacyclogenesis by insect urine components led us to test the effect of human urine as a culture additive. The addition of 1-5% urine to Schneider's Drosophila medium containing 10% foetal calf serum enhanced the growth of 11 Leishmania strains representing 8 different taxonomic groups. Cell division was stimulated in cultures with non-dividing organisms. Peak cell density was increased, as was the efficiency with which L. donovani could be isolated from infected hamsters. Preliminary work suggested that the modified medium would be useful for field isolation of L. donovani and L. braziliensis. The active nutrients or growth factors are not known.

引用

页码：477 / 479

页数：3

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