SENSITIVE DETERMINATION OF JOSAMYCIN AND ROKITAMYCIN IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION

被引：28

作者：

TOD, M

BIAREZ, O

NICOLAS, P

PETITJEAN, O

机构：

[1] Department of Pharmacotoxicology, Avicenne Hospital, 93000 Bobigny

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 575卷 / 01期

关键词：

D O I：

10.1016/0378-4347(92)80521-Q

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Rokitamycin and josamycin were successfully derivatized with dansylhydrazine in 20 min at 60-degrees-C. Rokitamycin and josamycin levels were determined in plasma after ion-pair extraction into hexane-isoamyl alcohol with lauryl sulphate and precolumn derivatization. Resolution was obtained by liquid chromatography with fluorescence detection (352/537 nm) in 12 min. The limit of detection was 20 ng/ml macrolide starting from 1 ml of plasma, and linearity was demonstrated between 50 and 400 ng/ml. Inter-run coefficients of variation were 10.2% at 100 ng/ml and 9.1% at 300 ng/ml. The system was reliably used for pharmacokinetic studies in plasma.

引用

页码：171 / 176

页数：6

共 11 条

[1] 1-DIMETHYLAMINONAPHTHALENE-5-SULFONYL HYDRAZINE (DANSYL HYDRAZINE) - FLUOROMETRIC REAGENT FOR CARBONYL COMPOUNDS [J].