ELECTROCHEMICAL IMMOBILIZATION OF ENZYMES .3. IMMOBILIZATION OF GLUCOSE-OXIDASE IN THIN-FILMS OF ELECTROCHEMICALLY POLYMERIZED PHENOLS

Electrochemically polymerized films of phenol and derivatives of phenol can be used to immobilize glucose oxidase in a thin layer at a platinum electrode. The immobilized enzyme retains its activity toward glucose oxidation, and hydrogen peroxide generated by the enzyme-catalyzed reaction of glucose with oxygen can be detected at 0.9 V vs SCE. The responses of the immobilized enzyme electrodes toward glucose are analyzed in terms of a thin-layer model. Of the five phenols employed (phenol, 3-nitrophenol, pyrogallol, 4-hydroxybenzenesulfonic acid, and bromophenol blue), phenol itself is found to be the most suitable.