A METHOD FOR QUANTITATIVE MEASUREMENT OF MITOCHONDRIAL CREATINE-KINASE IN HUMAN SKELETAL-MUSCLE

被引：20

作者：

SMEITINK, J

WEVERS, R

HULSHOF, J

RUITENBEEK, W

VONLITH, T

SENGERS, R

TRIJBELS, F

KORENKE, C

WALLIMANN, T

机构：

[1] UNIV HOSP NIJMEGEN, INST PAEDIAT, NIJMEGEN, NETHERLANDS

[2] UNIV HOSP NIJMEGEN, INST NEUROL, NIJMEGEN, NETHERLANDS

[3] SWISS FED INST TECHNOL, INST CELL BIOL, CH-8092 ZURICH, SWITZERLAND

来源：

ANNALS OF CLINICAL BIOCHEMISTRY | 1992年 / 29卷

关键词：

ENZYME EXTRACTION; CK ISOENZYMES; MITOCHONDRIAL MYOPATHY;

D O I：

10.1177/000456329202900213

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Defects in the mitochondrial energy generating system in patients with a mitochondrial myopathy are known to be localized in various enzyme complexes involved in energy production. Such a defect may exist at the level of mitochondrial creatine kinase (Mi-CK). On that account we have developed a method for measurement of the enzyme activity in human skeletal muscle biopsy material (> 10 mg). Interfering creatine kinase isoenzymes are removed by anion exchange and affinity chromatography. The activity of Mi-CK in reference skeletal muscle homogenates amounts to 240 +/- 88 mU/mg protein (30 +/- 8.0 mU/mg wet weight).

引用

页码：196 / 201

页数：6

共 16 条

[1] MITOCHONDRIAL CREATINE-KINASE ACTIVITY ALTERATIONS IN SKELETAL-MUSCLE DURING LONG-DISTANCE RUNNING [J].