ISOLATION OF AUTOPHAGOCYTOSIS MUTANTS OF SACCHAROMYCES-CEREVISIAE

被引：474

作者：

THUMM, M ^{[1
]}

EGNER, R ^{[1
]}

KOCH, B ^{[1
]}

SCHLUMPBERGER, M ^{[1
]}

STRAUB, M ^{[1
]}

VEENHUIS, M ^{[1
]}

WOLF, DH ^{[1
]}

机构：

[1] UNIV GRONINGEN, CTR BIOL, ELECTRON MICROSCOPY LAB, HAREN, NETHERLANDS

来源：

FEBS LETTERS | 1994年 / 349卷 / 02期

关键词：

YEAST; VACUOLE; AUTOPHAGOCYTOSIS; SCREENING PROCEDURE;

D O I：

10.1016/0014-5793(94)00672-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Protein degradation in the vacuole (lysosome) is an important event in cellular regulation. In yeast, as in mammalian cells, a major route of protein uptake for degradation into the vacuole (lysosome) has been found to be autophagocytosis. The discovery of this process in yeast enables the elucidation of its mechanisms via genetic and molecular biological investigations. Here we report the isolation of yeast mutants defective in autophagocytosis (aut mutants), using a rapid colony screening procedure.

引用

页码：275 / 280

页数：6

共 31 条

[1] AUSUBEL FM, 1987, CURR PROTOCOLS MOL B
[2] REGULATED IMPORT AND DEGRADATION OF A CYTOSOLIC PROTEIN IN THE YEAST VACUOLE
CHIANG, HL
SCHEKMAN, R
[J]. NATURE, 1991, 350 (6316) : 313 - 318
[3] PEPTIDE SEQUENCES THAT TARGET CYTOSOLIC PROTEINS FOR LYSOSOMAL PROTEOLYSIS
DICE, JF
[J]. TRENDS IN BIOCHEMICAL SCIENCES, 1990, 15 (08) : 305 - 309
[4] EGNER R, 1993, J BIOL CHEM, V268, P27269
[5] BIOGENESIS OF THE YEAST VACUOLE (LYSOSOME) - MUTATION IN THE ACTIVE-SITE OF THE VACUOLAR SERINE PROTEINASE YSCB ABOLISHES PROTEOLYTIC MATURATION OF ITS 73-KDA PRECURSOR TO THE 41.5-KDA PROENZYME AND A NEWLY DETECTED 41-KDA PEPTIDE
HIRSCH, HH
SCHIFFER, HH
MULLER, H
WOLF, DH
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1992, 203 (03): : 641 - 653
[6] BIOGENESIS OF THE YEAST VACUOLE (LYSOSOME) - PROTEINASE YSCB CONTRIBUTES MOLECULARLY AND KINETICALLY TO VACUOLAR HYDROLASE-PRECURSOR MATURATION
HIRSCH, HH
SCHIFFER, HH
WOLF, DH
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1992, 207 (03): : 867 - 876
[7] Hirsch HH., 1989, MOL CELL BIOL YEASTS, P134
[8] JONES EW, 1991, J BIOL CHEM, V266, P7963
[9] JONES EW, 1990, METHOD ENZYMOL, V185, P372
[10] JONES EW, 1991, METHOD ENZYMOL, V194, P428

← 1 2 3 4 →