A NOVEL STRONG PROMOTER OF THE GROEX OPERON OF SYMBIOTIC BACTERIA IN AMEBA-PROTEUS

被引：15

作者：

AHN, TI ^{[1
]}

LIM, ST ^{[1
]}

LEEU, HK ^{[1
]}

LEE, JE ^{[1
]}

JEON, KW ^{[1
]}

机构：

[1] UNIV TENNESSEE,DEPT ZOOL,KNOXVILLE,TN 37996

来源：

GENE | 1994年 / 148卷 / 01期

基金：

美国国家科学基金会;

关键词：

RECOMBINANT DNA; NUCLEOTIDE SEQUENCE; HEAT-SHOCK GENE; EVOLUTION OF ENDOSYMBIONT; EXPRESSION VECTORS;

D O I：

10.1016/0378-1119(94)90231-3

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Gram(-) symbiotic bacteria (called X-bacteria), present in the xD strain of Amoeba proteus as required cell components, contain a large amount of a 67-kDa protein, a GroEL analog. The complete nucleotide (nt) sequence of the groEx operon of X-bacteria has been determined and it has a high degree of nt identity with those of other bacterial groE operons. The groELx gene is expressed in transformed Escherichia coli and has a novel and potent promoter (P2) in addition to the heat-shock consensus promoter (P1). This is shown by the production of GroELx in Escherichia coli transformed with modified DNA clones lacking P1 and by an enhanced production of a GroELx::beta-galactosidase fusion protein when a portion of groEx containing P2 is linked to the lacZ gene. Primer-extension analyses revealed the presence of possible P2 sequences within the open reading frame of the groESx gene. It is suggested that the presence of a potent P2 in the X-bacterial gene is an adaptation for the endosymbiotic bacteria to survive within a potentially hostile intracellular environment.

引用

页码：43 / 49

页数：7

共 40 条

[1] ANALYSIS OF PROTEINS SYNTHESIZED BY SALMONELLA-TYPHIMURIUM DURING GROWTH WITHIN A HOST MACROPHAGE [J].