EXTRACTION OF HIGH-QUALITY DNA FROM BLOODSTAINS USING DIATOMS

被引：10

作者：

GUNTHER, S ^{[1
]}

HEROLD, J ^{[1
]}

PATZELT, D ^{[1
]}

机构：

[1] UNIV WURZBURG,INST VIROL,D-97078 WURZBURG,GERMANY

来源：

INTERNATIONAL JOURNAL OF LEGAL MEDICINE | 1995年 / 108卷 / 03期

关键词：

DNA EXTRACTION; DIATOMS; PCR; STAIN ANALYSIS;

D O I：

10.1007/BF01844828

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

A simple method is described for the extraction of high quality DNA for PCR amplification. The DNA was extracted by using Chelex-100 ion exchange resin or a special cell lysis buffer containing proteinase K. For further purification the DNA was bound to silica in the presence of a chaotrophic agent. Hence it is possible to unlimitedly wash the bound DNA and inhibitory substances are removed. By using diatoms as a source of silicates, this method is very economical and can therefore be used as a routine method.

引用

页码：154 / 156

页数：3

共 10 条

[1]

ALFORD RL, 1994, AM J HUM GENET, V55, P190

[2]

ALLEN RC, 1991, BIOTECHNIQUES, V7, P736

[3] RAPID AND SIMPLE METHOD FOR PURIFICATION OF NUCLEIC-ACIDS [J].