CLONING AND MAPPING OF THE U2AF1-RS2 GENE WITH A HIGH TRANSMISSION DISTORTION IN INTERSPECIFIC BACKCROSS PROGENY

We have cloned and analyzed the mouse U2af1-rs2 gene based on its sequence similarity to the imprinted gene U2af1-rs1 (SP2). Sequence analysis of this U2af1-rs2 cDNA revealed that it contained an open reading frame encoding a protein of 462 amino acid residues. The predicted amino acid sequence showed 72.7 and 35.8% identity to the U2af1-rs1 and U2 small nuclear ribonucleoprotein auxiliary factor, respectively. Interspecific backcross analysis showed this gene to map to the distal region of the X chromosome and also indicated that there was significant distortion of transmission ratio of the U2af1-rs2 allele in the backcrossed progeny from (C57BL/6J X Mus spretus)F-1 females mated to Mus spretus males. (C) 1995 Academic Press, Inc.