FRACTIONATION OF TRANSFER RIBONUCLEIC-ACIDS BY CHROMATOGRAPHY ON NEUTRAL POLYSACCHARIDE MEDIA IN REVERSE SALT GRADIENTS

被引：9

作者：

MORRIS, CJOR

机构：

[1] Department of Experimental Biochemistry, London Hospital Medical College, Queen Mary College, London, E1 4NS, Mile End Rd

来源：

JOURNAL OF CHROMATOGRAPHY | 1978年 / 159卷 / 01期

关键词：

D O I：

10.1016/S0021-9673(00)98544-3

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A sequential scheme for the fractionation of yeast transfer RNAs by chromatography on the polysaccharide gel media Sepharose 4B and Ultrogel AcA 44 in reversed concentration gradients of ammonium sulphate is described and the optimum conditions worked out. Four species, tRNAMet, tRNAAsn, tRNAPro and tRNAAla are obtained in a highly purified form, while several other acceptor species are highly concentrated in the subfractions. The mechanism of this chromatographic system is discussed. © 1978.

引用

页码：33 / 46

页数：14

共 13 条

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MORRIS CL, UNPUBLISHED

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