STRUCTURE-FUNCTION ANALYSIS OF HUMAN INTERLEUKIN-6 - EVIDENCE FOR THE INVOLVEMENT OF THE CARBOXY-TERMINUS IN FUNCTION

被引：73

作者：

KRUTTGEN, A ^{[1
]}

ROSEJOHN, S ^{[1
]}

MOLLER, C ^{[1
]}

WROBLOWSKI, B ^{[1
]}

WOLLMER, A ^{[1
]}

MULLBERG, J ^{[1
]}

HIRANO, T ^{[1
]}

KISHIMOTO, T ^{[1
]}

HEINRICH, PC ^{[1
]}

机构：

[1] OSAKA UNIV, INST MOLEC & CELLULAR BIOL, SUITA, OSAKA 565, JAPAN

来源：

FEBS LETTERS | 1990年 / 262卷 / 02期

关键词：

Biological activity; Carboxy-terminus; Circular dichroism; Deletion mutant; Interleukin-6; Secondary structure prediction;

D O I：

10.1016/0014-5793(90)80219-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

C-terminally deleted analogs of human interleukin-6 (IL-6) have been constructed at the cDNA level, and after cell-free transcription and translation their biological activity was analyzed. Removal of only 4 amino acids resulted in complete loss of biological activity as determined by the B9 cell proliferation assay. Secondary structure prediction of human IL-6 resulted in 58% helix, 14% β-structure, and 28% turn and coil (average of 3 independent methods). The circular dichroism of recombinant human IL-6 was measured in the near and far UV. Evaluation of the latter in terms of secondary structures gave 67% helix, 15% β-structure, and 18% turn and coil. © 1990.

引用

页码：323 / 326

页数：4

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