THE CDNAS CODING FOR THE ALPHA-SUBUNIT AND BETA-SUBUNIT OF XENOPUS-LAEVIS CASEIN KINASE-II

被引：36

作者：

JEDLICKI, A

HINRICHS, MV

ALLENDE, CC

ALLENDE, JE

机构：

[1] UNIV CHILE, FAC MED, DEPT BIOQUIM, CASILLA 70086, SANTIAGO 7, CHILE

[2] UNIV CHILE, FAC CIENCIAS, DEPT BIOL, SANTIAGO 7, CHILE

来源：

FEBS LETTERS | 1992年 / 297卷 / 03期

关键词：

PROTEIN KINASE; AMPHIBIAN OOCYTE; PROTEIN PHOSPHORYLATION; SEQUENCE MOTIF; XENOPUS-LAEVIS; CASEIN KINASE-II;

D O I：

10.1016/0014-5793(92)80556-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using a lambda-gt10 cDNA library obtained from Xenopus laevis oocytes and probes derived from the known sequences of the human and Drosophila genes, a cDNA coding for the alpha-subunit of the X. laevis casein kinase II was isolated. The coding sequence of this clone determines a polypeptide of 350 amino acids. The X. laevis sequence is 98% identical to the human and rat proteins in the first 323 amino acids. Using the polymerase chain reaction to generate a 370 nucleotide-long probe, it was possible to clone and sequence a cDNA of 900 nucleotides that coded for the X. laevis beta-subunit of casein kinase II. The derived sequence is 215 amino acids long and again shows an extraordinary degree of conservation with other species.

引用

页码：280 / 284

页数：5

共 33 条

[1] STIMULATION OF CASEIN KINASE-II BY EPIDERMAL GROWTH-FACTOR - RELATIONSHIP BETWEEN THE PHYSIOLOGICAL-ACTIVITY OF THE KINASE AND THE PHOSPHORYLATION STATE OF ITS BETA-SUBUNIT [J].