IDENTIFICATION OF AN AII(3-8) [AIV] BINDING-SITE IN GUINEA-PIG HIPPOCAMPUS

被引:153
作者
HARDING, JW
COOK, VI
MILLERWING, AV
HANESWORTH, JM
SARDINIA, MF
HALL, KL
STOBB, JW
SWANSON, GN
COLEMAN, JKM
WRIGHT, JW
HARDING, EC
机构
[1] WASHINGTON STATE UNIV, DEPT PHARMACOL, PULLMAN, WA 99164 USA
[2] WASHINGTON STATE UNIV, DEPT PHYSIOL, PULLMAN, WA 99164 USA
[3] WASHINGTON STATE UNIV, DEPT PSYCHOL, PULLMAN, WA 99164 USA
关键词
ANGIOTENSIN; ANGIOTENSIN-II(3-8); HIPPOCAMPUS; RECEPTOR; GUINEA PIG;
D O I
10.1016/S0006-8993(10)80047-2
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
A unique angiotensin binding site specific for the hexapeptide, AII(3-8), has been identified in guinea pig hippocampus. This binding site, which is present in the pyramidal cell layer of CA1, CA2, CA3 of the hippocampus and dentate gyrus, binds AII(3-8) with high affinity (K(D) = 1.29+/-0.18 nM) in a saturable manner (B(max) = 449+/-62 fmol/mg protein). The N-terminal structure of the binding ligand is paramount in determining the binding affinity. The C-terminal requirements seem less stringent as evidenced by the binding affinity of AII(3-7) (K(D) = 20.9+/-2.1 nM). Neither AII, AIII, Sar1,Ile8-AII, Dup 753 nor CGP42112A appear to bind, indicating that this binding site is neither the AT1 nor AT2 sites described for AII/AIII. Autoradiographic analysis of hippocampus binding confirms the inability of Sar1,Ile8-All to compete for [I-125]AII(3-8) binding. Conversely AII(3-8) was unable to displace [I-125]Sar1,Ile8-AII binding.
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页码:340 / 343
页数:4
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