DETERMINATION OF COPPER(II) CHLOROPHYLLIN BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：47

作者：

INOUE, H ^{[1
]}

YAMASHITA, H ^{[1
]}

FURUYA, K ^{[1
]}

NONOMURA, Y ^{[1
]}

YOSHIOKA, N ^{[1
]}

LI, S ^{[1
]}

机构：

[1] BEIJING UNIV,DEPT TECHNOL PHYS,BEIJING 100871,PEOPLES R CHINA

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 679卷 / 01期

关键词：

D O I：

10.1016/0021-9673(94)80315-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Copper(II) chlorophyllin, consisting of copper(II) pheophorbide a, copper(II) chlorin e(6), copper(II) rhodin g(7) and copper(II) chlorin e(4), was prepared and separated by semi-preparative high-performance liquid chromatography (HPLC). The components of copper(II) chlorophyllin were determined on a reversed-phase Inertsil ODS-2 column using a mobile phase of methanol-water (97:3, v/v) containing 1% (v/v) of acetic acid. Linear calibration plots were obtained for copper(II) chlorophyilin in the concentration range of 0-30 mu g cm(-3) with photometric detection at 407 or 423 nm. The detection limits of copper(II) pheophorbide a, copper(II) chlorin e(6), copper(II) rhodin g(7) and copper(II) chlorin e(4) were 3.5, 1.5, 3.3 and 1.4 ng cm(-3) with relative standard deviations (n = 10) of 1.8, 1.6, 5.2 and 3.6%, respectively. The reversed-phase HPLC method proposed here was demonstrated to be useful for the determination of the components of sodium copper(II) chlorophyllin.

引用

页码：99 / 104

页数：6

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