COMPETITIVE-INHIBITION OF NEUROSPORA-CRASSA CHITIN SYNTHETASE-ACTIVITY BY TUNICAMYCIN

被引：8

作者：

SELITRENNIKOFF, CP ^{[1
]}

机构：

[1] MERCK INST THERAPEUT RES, DEPT BAS MICROBIOL, RAHWAY, NJ 07065 USA

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1979年 / 195卷 / 01期

关键词：

D O I：

10.1016/0003-9861(79)90346-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Chitin synthetase from Neurospora crassa was inhibited in vitro by tunicamycin. The drug was found to be kinetically a linear competitive inhibitor (Ki ∼ 480 μm) with respect to the substrate, UDP-N-acetylglucosamine. Since tunicamycin and UDP-N-acetylglucosamine are structurally similar and there exists linear competitive inhibition, it is likely that tunicamycin inhibits enzyme activity by directly competing with the substrate for access to the enzyme. © 1979.

引用

页码：243 / 244

页数：2

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