HOMOGENEOUS LATEX IMMUNOASSAY FOR THYROID-HORMONE TESTING - DETERMINATION OF THYROXINE AND TRIIODOTHYRONINE

被引：3

作者：

MARESCHAL, JC ^{[1
]}

CAMBIASO, CL ^{[1
]}

JANSSENS, J ^{[1
]}

LIMET, JN ^{[1
]}

TASIAUX, N ^{[1
]}

COLLETCASSART, D ^{[1
]}

机构：

[1] UNIV CATHOLIQUE LOUVAIN,FAC MED,EXPTL MED UNIT,75 AVE HIPPOCRATE,B-1200 BRUSSELS,BELGIUM

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1990年 / 131卷 / 01期

关键词：

Latex agglutination; Latex immunoassay; Non-isotopic immunoassay; Thyroid hormone;

D O I：

10.1016/0022-1759(90)90243-O

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe here two latex immunoassays for total thyroxine (T4) and total triiodothyronine (T3). These homogeneous 60 min assays are quantified by optically counting the monomeric particles remaining after agglutination. When precision is assessed, both methods display coefficients of variation of 3-7% for within-run assays and 4-10% for between-run assays. The accuracy of the methods, as tested by dilution and spike recovery experiments, was found to be satisfactory. Two correlation studies were carried out to compare the present method with leading commercial methods. The coefficients obtained were: r = 0.92 and r = 0.93 with 150 sera for T3, and r = 0.95 (150 sera and r = 0.93 (108 sera) for T4. © 1990.

引用

页码：137 / 142

页数：6

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